Analysis of Active Substances in Kratom (Mitragyna speciosa Korth.) Cocktail
Chittrakarn, Somsmorn; Penchumruth, Pimpimon; Krewpradub, Niwat Prince of Songkla University, Thailand; Scientific Equipment Center, Prince of Sonkla University, Thailand; Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Thailand
Kratom leaves (Mitragyna speciosa Korth.) are usually chewed by individuals to improve their tolerance to work and relieve muscle strains. The pattern of use has changed from chewing to drinking the kratom cocktail. The kratom cocktail (dubbed 4x100) is the mixture of boiled kratom leaves, coke, antitussis syrup, coffee, or codeine. Mitragynine is the major alkaloid in kratom leaves.
The aim of this study was to investigate the qualitative and quantitative analysis of active substances in kratom cocktail such as mitragynine, caffeine, codeine, chlorpheniramine, and phenylephrine.
Analysis of the substances in kratom cocktail was performed by high-performance liquid chromatography using isocratic elution and diode array detector. The correlation coefficient (r2) of standard mitragynine, codeine, caffeine, chlorpheniramine, and phenylephrine was 0.9957, 0.9995, 0.9997, 0.9994, and 0.9993, respectively. The limit of detection of these active
substances was 1, 0.005, 0.005, 0.2, and 0.2 mg/L, respectively. The limit of quantification (LOQ) was 3, 0.01, 0.01, 0.4, and 0.4 mg/L, respectively. The
quantitative analysis of mitragynine, codeine, caffeine, chlorpheniramine, and phenylephrine in kratom cocktail was 89.3124, 606.1617, 468.4403 mg/L,
The kratom cocktail sample had mitragynine (89.3124 mg/L). The extraction of 100 g kratom leaves contained the constituent of mitragynine 127 mg.
Thus mitragynine in kratom cocktail was less than in 100 g kratom leaves. Codeine and caffeine were contained in very high concentrations (606.1617 and
468.4403 mg/L, respectively). Other substance such as antihistamines or decongestants were not found. The addictive effect of kratom cocktail should be
further investigated in an animal model.